电离辐射通过转化生长因子-β-介导的上皮-粘液转换来促进癌细胞的侵袭迁移

Int J Radiat Oncol Biol Phys 2011 Dec;81 (5): 1530-7. [IF:4.503]Ionizing radiation promotes migration and invasion of cancer cells through transforming growth factor-Beta-mediated epithelial-mesenchymal transition.Zhou YC , Liu JY , Li J , Zhang J , Xu YQ , Zhang HW , Qiu LB , Ding GR , Su XM , Mei-Shi , Guo GZ .Department of Radiation Oncology, Xijing Hospital Fourth Military Medical University, Xi'an, China; Department of Radiation Medicine, College of Preventive Medicine, Xijing Hospital Fourth Military Medical University, Xi'an, China.第四军医大学西京医院放射科

AbstractTo examine whether ionizing radiation enhances the migratory and invasive abilities of cancer cells through transforming growth factor (TGF-β)-mediated epithelial-mesenchymal transition (EMT). Six cancer cell lines originating from different human organs were irradiated by (60)Co γ-ray at a total dose of 2 Gy, and the changes associated with EMT, including morphology, EMT markers, migration and invasion, were observed by microscope, Western blot, immunofluorescence, scratch assay, and transwell chamber assay, respectively. Then the protein levels of TGF-β in these cancer cells were detected by enzyme-linked immunosorbent assay, and the role of TGF-β signaling pathway in the effect of ionizing radiation on EMT was investigate by using the specific inhibitor SB431542. After irradiation with γ-ray at a total dose of 2 Gy, cancer cells presented the mesenchymal phenotype, and compared with the sham-irradiation group the expression of epithelial markers was decreased and of mesenchymal markers was increased, the migratory and invasive capabilities were strengthened, and the protein levels of TGF-β were enhanced. Furthermore, events associated with EMT induced by IR in A549 could be reversed through inhibition of TGF-β signaling. These results suggest that EMT mediated by TGF-β plays a critical role in IR-induced enhancing of migratory and invasive capabilities in cancer cells.

简要 ：探讨强磁场是否可通过转化生长因子-β（TGF-β）-内皮细胞的上皮复合物质-间质匹配 (EMT)来作出贡献恶性肿瘤的蹂躏迁移。适用总量2Gy(60)Coγ中央线紫外中央线照射源自人类所器官的6种恶性肿瘤，记录与EMT相关的波动，这还包括分别能用光学仪器技术，复合物质印迹工具，免疫荧光技术，划痕试验和Transwell张以试验来仔细观察并侦测复合物质组织形态，EMT标示，蹂躏迁移并能等。换用复合物联免疫吸附法侦测这些恶性肿瘤之前TGF-β复合物技术水平，能用特别类固醇SB431542来评估TGF-β波形移动式在强磁场EMT之前的作用。经过总量为2Gy紫外中央线照射的恶性肿瘤之前存在间叶复合物质的表示，与假紫外中央线照射组相比其上皮复合物质标示缩减，间叶复合物质标示增加，同时其蹂躏移出并能强化，TGF-β复合物技术水平也提高。进一步发现由A549强磁场诱导的EMT可通过对TGF-β波形选择性发生反败为胜。这些结果表明TGF-β内皮细胞的EMT在强磁场诱导强化恶性肿瘤蹂躏移出并能之前起着关键作用。

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